Bovine Leukocyte Adhesion Deficiency in Danish Holstein-Friesian Cattle
نویسندگان
چکیده
منابع مشابه
Detection of New Silent Mutation at 348 bp Position in a CD18 Gene in Holstein Cattle Normal and Heterozygous for Bovine Leukocyte Adhesion Deficiency Syndrome
In India, Holstein and its crosses are being used extensively in breeding programmes and all these breeding bulls are screened for autosomal recessive genes. Blood samples are collected in ethylenediaminetetraacetic acid (EDTA) coated tubes and DNA was isolated by using phenol-chloroform method. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) wereperformed by using...
متن کاملInvestigation of Bovine Leukocyte Adhesion Deficiency (BLAD) and Complex Vertebral Malformation (CVM) in a Population of Iranian Holstein Cows
In the present research, molecular detection of bovine leukocyte adhesion deficiency (BLAD) and complex vertebral malformation (CVM)in a population of Iranian Holstein cows has been carried outusing milk somatic cells by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The BLAD and CVM are monogenic and autosomal recessive heredity lethal syndrome in Holstein-Friesi...
متن کاملAlimentary and respiratory tract lesions in eight medically fragile Holstein cattle with bovine leukocyte adhesion deficiency (BLAD).
Lesions in the alimentary tract were studied in eight medically fragile Holstein cattle homozygous for the bovine leukocyte adhesion deficiency (BLAD) allele as determined by polymerase chain reaction and restriction endonuclease analysis. These cattle received institutional medical care but died or were euthanatized because of chronic debilitation associated with diarrhea (6/8) and pneumonia (...
متن کاملIdentification and prevalence of a genetic defect that causes leukocyte adhesion deficiency in Holstein cattle.
Two point mutations were identified within the gene encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency (LAD). One mutation causes an aspartic acid to glycine substitution at amino acid 128 (D128G) in the highly conserved extracellular region of this adhesion glycoprotein, a region where several mutations have been found to cause human LAD. The other mutation is...
متن کاملDevelopment of a fast and economical genotyping protocol for bovine leukocyte adhesion deficiency (BLAD) in cattle
Fast and economical means of assaying SNP's are important in diagnostic assays, especially when a large number of animals have to be screened for a genetic disease. This study was aimed at the development of a fast and economical screening assay for bovine leukocyte adhesion deficiency (BLAD) which is an important genetic disease of cattle industry. Four primers were designed where the outer pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Veterinaria Scandinavica
سال: 1993
ISSN: 1751-0147
DOI: 10.1186/bf03548187